ಐಸೊಫ್ಲುರೇನ್, as a classic and effective active drug for inhalation anesthesia, has played an important role in mouse experiments. ಐಸೊಫ್ಲುರೇನ್ is undoubtedly one of the most ideal anesthetic drugs in animal experiments at present because of its advantages of rapid onset, strong controllability and little physiological influence.
What Will Happen to Mice After Using Isoflurane?
Reduce physiological interference
Another advantage of mouse anesthesia isoflurane is that it has less interference with physiological system. Compared with other types of anesthetics, isoflurane has little effect on physiological indexes such as heart, respiratory system and body temperature in mice. During anesthesia, isoflurane does not cause severe blood pressure drop or abnormal heart rate, and the respiratory frequency of mice changes smoothly, which provides more control space for researchers who need to observe the physiological reaction of mice or conduct long-term experiments.
Suitable for long-term experiments
Some experiments in neuroscience, pharmacology and toxicology need to anesthetize mice for a long time, and isoflurane just meets this requirement. In the long-term experiment, the experimenter needs to ensure that the mice maintain a stable anesthesia state and reduce the anesthesia interference in the experiment. ಐಸೊಫ್ಲುರೇನ್, through its good metabolic characteristics and stability, enables mice to remain anesthetized for a long time and recover quickly after anesthesia stops, which is especially important for many experiments that need long-term continuous observation.
Matters Needing Attention in Isoflurane Anesthesia in Mouse Experiment
Although isoflurane has many advantages in mouse anesthesia, its use still needs scientists' careful attention and strict control. The process of anesthesia involves many links, and the negligence of each link may affect the experimental results and even cause unnecessary pain or injury to animals. Therefore, the following points need special attention:
Control of anesthetic concentration
The anesthetic effect of isoflurane is closely related to its concentration. Too high anesthetic concentration may lead to too deep anesthesia in mice, and even lead to respiratory depression, cardiac arrest and other risks. However, if the concentration is too low, it may lead to insufficient anesthesia, which can not effectively inhibit the activity and pain of mice, thus affecting the experimental results. In order to ensure the accurate control of anesthesia, experimenters need to make a reasonable anesthesia plan according to the weight, age and health status of mice.
Grasping anesthesia time
Anesthesia time is also an important factor affecting the experimental results. In some experiments, long-term anesthesia may affect the physiological system of mice, such as hypothermia, abnormal breathing frequency, etc., so the anesthesia time needs to be accurately grasped according to the experimental requirements. In order to prevent the anesthesia time from being too long or too short, experimenters usually need to monitor the physiological indexes of mice in real time, such as body temperature, respiratory rate and heart rate, and adjust the anesthesia concentration or suspend anesthesia as needed.
Preparation before and after anesthesia
Preparation before and after anesthesia is equally important. Before anesthesia, experimenters need to ensure the normal operation of anesthesia equipment and check the concentration and quality of anesthetic drugs; After anesthesia, experimenters need to ensure that the recovery environment of mice is safe and comfortable, such as providing mice with suitable temperature and clean recovery cages, and observing their physiological reactions during recovery. If there are abnormal conditions, such as delayed recovery and dyspnea, the experimenter should take corresponding measures immediately, such as oxygen support and drug intervention.