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Pharmaceutical intermediates are vital chemical compounds that serve as building blocks in the synthesis of active pharmaceutical ingredients (APIs). These intermediates are crucial in the drug manufacturing process and play a significant role in the pharmaceutical supply chain. They bridge the gap between raw materials and final active ingredients, making them essential in the production of medicines.


Active Pharmaceutical Ingredients (APIs) are crucial components in the field of pharmacy, serving as the biologically active elements in medications that produce the desired therapeutic effect. The significance of APIs cannot be overstated; they are the backbone of pharmaceutical formulations, and their quality directly affects the efficacy and safety of medicines.


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Typically, SDS-PAGE (polyacrylamide gel electrophoresis) is the most commonly used method for separating proteins. The resulting gel can be stained to visualize the separated proteins, allowing researchers to analyze the composition of samples, estimate molecular weights, and assess purity.


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