In the field of scientific research, animal experiments have long been an indispensable means to explore biomedicine, pharmacology, neuroscience and other disciplines. Especially in the use of mouse models, mice have become one of the most commonly used animals in experimental research because of their rapid reproduction, thorough genome analysis and good simulation in many disease models. Among many anesthetic drugs, Isoflurane, as an inhalation anesthetic active drug, has been widely used in the anesthesia of experimental animals such as mice because of its remarkable anesthetic effect and little influence on animals.
Pharmacological Characteristics of Isoflurane
Isoflurane is a widely used active pharmaceutical ingredient, which belongs to haloalkane anesthetic. Its molecular structure makes it have the advantages of rapid onset, strong control and relatively little influence on physiological system. Isoflurane is a colorless and odorless liquid at room temperature. After inhalation, isoflurane can quickly enter the blood through the respiratory tract and produce anesthetic effect.
The pharmacological mechanism of isoflurane mainly achieves anesthetic effect by interacting with different receptors in the nervous system and inhibiting nerve transmission.
It can enhance the inhibitory effect of neurotransmitter γ -aminobutyric acid (GABA) and inhibit the action of excitatory neurotransmitters such as glutamic acid, which leads to the decrease of neural activity in cerebral cortex and spinal cord, making individuals unconscious and unable to feel pain.
Isoflurane is an ideal anesthetic in animal experiments because of its rapid inhalation and metabolism. By accurately controlling the anesthetic concentration, the experimenter can maintain the physiological balance of mice under anesthesia and quickly restore their awake state, thus reducing the interference of the experiment to animals.
Application of Isoflurane in Anesthesia of Mice
Mouse is one of the commonly used experimental animals in laboratory, which is widely used in basic and applied research in pharmacology, toxicology, genetics, immunology, neuroscience and other fields. Anesthesia is an inevitable step in mouse experiments, because anesthesia can ensure that animals remain stable during the experiment, reduce their activities and pain, and avoid physiological stress caused by pain, thus making the experimental results more reliable.
Rapid onset and recovery
One of the biggest advantages of isoflurane is its quick onset time and recovery time. Isoflurane provides great convenience for experiments that need to accurately control the depth of anesthesia. After mice are exposed to isoflurane, the anesthetic effect usually appears within a few minutes, and they quickly recover to the sober state after the anesthetic concentration is reduced or stopped. The recovery speed of anesthesia is especially important for scientific experiments, because in the process of anesthesia, the experimenter hopes to interrupt the experiment as little as possible to ensure the continuity and accuracy of the data.
Precise control of anesthesia depth
Isoflurane is a gas anesthetic, and its anesthetic depth can be accurately controlled by adjusting its concentration. By adjusting the flow and concentration of anesthesia machine, experimenters can maintain mice in different states such as mild anesthesia and deep anesthesia to meet the needs of different experiments. Compared with injecting anesthetic drugs, isoflurane can adjust the depth of anesthesia more carefully, avoid the situation of excessive anesthesia or insufficient anesthesia, and reduce the risks related to anesthesia.